【LWnt-3A細(xì)胞】小鼠皮下結(jié)締組織細(xì)胞
細(xì)胞名稱：小鼠皮下結(jié)締組織細(xì)胞；LWnt-3A
組織來源：皮下結(jié)締組織細(xì)胞
培養(yǎng)條件：【LWnt-3A細(xì)胞】小鼠皮下結(jié)締組織細(xì)胞DMEM-H:Dulbecco'sModifiedEagle'sMedium(DMEH-214.5g/LiterGlucose)胎牛血清，10%；0.4mg/mlG-418
形態(tài)：貼壁；成纖維細(xì)胞
細(xì)胞來源：：L-M(TK-)cells(ATCCCCL-1.3)用Wnt-3A表達(dá)載體轉(zhuǎn)染并在含G418的培養(yǎng)基中篩選穩(wěn)轉(zhuǎn)株。Wnt-3A基因編碼一個(gè)有變異的信號(hào)功效分泌性糖蛋白。Wnt基因控制胚胎發(fā)過程中的許多模式形成和生長(zhǎng)事件。這些細(xì)胞分泌有生物活性的Wnt-3A蛋白。它們是目前生產(chǎn)Wnt-3A條件培養(yǎng)基的來源。由于這種條件培養(yǎng)基還包含Wnt-3A蛋白外的其他因子，對(duì)于涉及Wnt-3A條件培養(yǎng)基的實(shí)驗(yàn)有必要用其來源細(xì)胞株(ATCCCRL-2648)作對(duì)照。
我們有細(xì)胞庫(kù)和專業(yè)實(shí)驗(yàn)室無菌操作，同時(shí)接受客戶復(fù)蘇、委托培養(yǎng)服務(wù)，并提供的科研項(xiàng)目解決方案以及實(shí)驗(yàn)服務(wù)。購(gòu)買我司細(xì)胞的客戶請(qǐng)先與公司細(xì)胞銷售人員核實(shí)訂購(gòu)的細(xì)胞株名稱、種屬、貨號(hào)、數(shù)量、協(xié)商細(xì)胞價(jià)格并預(yù)約發(fā)貨期與提取方式。向銷售人員索取細(xì)胞株說明書并認(rèn)真閱讀以方便你的實(shí)驗(yàn)操作。
對(duì)于培養(yǎng)，只要我們細(xì)心操作，注意細(xì)節(jié)，并不是大家說的那樣困難。對(duì)于有經(jīng)驗(yàn)和有條件的客戶，我們提供專人指導(dǎo)。對(duì)于無培養(yǎng)經(jīng)驗(yàn)和條件的客戶，建議由公司代為培養(yǎng)細(xì)胞及進(jìn)行后續(xù)研究。我司對(duì)每一位客戶追蹤服務(wù)，因材施教，對(duì)產(chǎn)品不僅售前負(fù)責(zé)，售后更負(fù)責(zé)。
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